Journal: Neoplasia (New York, N.Y.)

Article Title: Bcl-2 and N-Myc Coexpression Increases IGF-IR and Features of Malignant Growth in Neuroblastoma Cell Lines

doi:

Figure Lengend Snippet: Microvessel counts of tumors. Two unique SH-EP1 and SK-N-AS transfected cell lines expressing N-Myc and Bcl-2 each formed tumors in nude mice. At sacrifice, tumors were resected and microvessels quantified by CD31 expression. Data presented as mean vessels/10 HPF.

Article Snippet: Slides were incubated with primary antibody (rabbit antimouse CD31, 1:10, Dako, Carpinteria, CA) overnight at 37°C, then with secondary antibody (biotinylated goat antirabbit IgG, 1:500) for 30 minutes, followed by a 30-minute incubation with avidin-biotin-horseradish peroxidase complex (ABC, Vector Laboratories, Burlingame, CA).

Techniques: Transfection, Expressing